Sanger Sequencing at Eurofins Genomics

Mix2Seq Service

Fastest Sanger sequencing of premixed samples in tubes.

Experience ultimate innovation and highest convenience in Sanger sequencing.

Standard primer

SeqPrimer

Mix2seq kits

Highlights of the Mix2Seq Service:

Ultimate innovation

Buy one Mix2Seq Kit to pay for 96 reactions
Share your Mix2Seq data with others via your group
Transfer Mix2Seq Kits with all rights to someone else
Name your samples under My Kits & Labels

Highest convenience

Every Mix2Seq Kit includes 96 barcoded tubes and lids
Just provide the tubes - no online ordering necessary
Send us your samples using our free pick-up service
Use our sample bags and boxes to send your samples
Make use from our pre-defined Standard Primers

Excellent quality & service

Data delivery until 12 pm CET next business day with Mix2Seq NightXpress
Data delivery until 6 pm CET next business day with the regular Mix2Seq service.
Obtain reading lengths of up to 1100 high quality bases
Sequencing data are provided in the following file formats by default:
- .ab1 files - ABI trace files with all raw data
- .scf files -Trace files with quality values
- .seq files - Clipped & unclipped FASTA sequences
- .phd.1 files - With base call and quality information
- .pdf quality report - Trace file with quality scores
- FASTA file with all sequences

>> Request Free Trial

>> FAQ's Sanger Sequencing

>> SEQuencing Primers

>> standard primer

Related Information

Sample Preparation & Submission

4 little steps to optimum results

	Your template should consist of 5 µl purified DNA in either of the following concentrations: Plasmid DNA up to 15kbp: 50-100 ng/µl PCR products: 150-300 bp: 1 ng/µl; 300-1000 bp: 5 ng/ µl; 1000 - 3000 bp 10 ng/µl
	Add 5 µl of your SeqPrimer with a concentration of 5 pmol/µl (5 µM).
	Ensure that the total volume of your DNA / primer mix is at least 10 µl.
	Pipet your premixed sample in one of the Mix2Seq tubes and seal the tube with the blue or yellow lid from the enclosed cap pad.

Do not forget to note the barcode number on the side of the tubes before you send us your samples!

Conditions

DNA templates must be purified. Sequencing reactions cannot be repeated and all reactions will be charged. Samples are not stored. For samples containing difficult-to-read streches (GC-rich, hairpin structurs...) we recommend our TubeSeq Service.