NGS UDI Primer Sets

Eliminate Index Misassignment. Sequence with Confidence.

Ready-to-use Unique Dual Index primer sets for Illumina workflows with ultra-low cross-contamination and maximum demultiplexing accuracy.

• 4 sets of 96 unique UDI primer pairs

• Up to 384 index combinations per lane

• In-stock ready-to-use plates

• ISO 13485 certified

Built for Accurate Multiplex Sequencing

Multiplexing reduces sequencing costs and increases throughput. But poor index design, index hopping and cross-contamination can lead to misassigned reads and unreliable results. Our NGS UDI Primer Sets are specifically designed to deliver accurate sample identification and confident sequencing analysis. The produktion process was validated and are used by our inhouse NGS experts.

Product Selection

	NGS UDI Primer Set 96-01 UDP0001-0096 96 primer pairs for 96 reactions Click here to download plate assignment with u7 and u5 index primer sequences.*	Quantity Price N/A*


	NGS UDI Primer Set 96-02 UDP0097-0192 96 primer pairs for 96 reactions Click here to download plate assignment with u7 and u5 index primer sequences.*	Quantity Price N/A*


	NGS UDI Primer Set 96-03 UDP0193-0288 96 primer pairs for 96 reactions Click here to download plate assignment with u7 and u5 index primer sequences.*	Quantity Price N/A*


	NGS UDI Primer Set 96-04 UDP0289-0384 96 primer pairs for 96 reactions Click here to download plate assignment with u7 and u5 index primer sequences.*	Quantity Price N/A*

* For information on which u5 index orientation to use (forward or reverse complement), please refer to the following Illumina page:
https://support-docs.illumina.com/SHARE/AdapterSequences/Content/SHARE/AdapterSeq/Overview.htm

Information for the use of 12 bp UDI sequences for sequencing on MiSeq systems can be found here.

Why Choosing Eurofins Genomics NGS UDI Primer Sets

🎯 Reliable Results. No Compromises.

✅ 12-base UDIs

✅ ≥4 base edit distance

✅ 99.9986% correct read assignment

✅ Validated in-house on NovaSeq 6000

✅ Ready-to-use format

🧬 Built for Modern NGS Workflows

✅ RNA Sequencing

✅ Oncology Panels

✅ Targeted Sequencing

✅ Microbiome Research

✅ Large Multiplex NGS Projects

📈 Designed for Scalability

✅ 96 unique index pairs per set

✅ Combine four sets

✅ Up to 384 unique UDI combinations

✅ Compatible with large projects

Feature	Typical NGS UDI Sets	Eurofins Genomics NGS UDI Primer Sets
Index Length	8-10 bp	12 bp
Edit Distance	Variable	≥ 4 bp
Read Misassignment Risk	Not tested / Variable	< 0.4 reads / 10M reads
Scalability	Variable	Up to 384 UDIs
In-house NGS Validation	Rarely available	✅

Product Specifications

What you can expect from our NGS UDI Primer Sets

Feature	Details
Format	96-well plate (96 primer pairs)
Primer Concentration	Each primer pair is supplied at 10 µM in 10 µL bidestilled water and is intended for single-use applications
Index length	12 nucleotides
Edit distance	4 bases
Compatibility	Compatible with Illumina TruSeq & NEB adapters
Quality	NGS performance-verified in-house & ISO 13485 medical device certification
Cross-contamination	Average risk of misassigned reads between two UDI pairs of < 0.4 reads when sequencing 10M reads
Purification	Proprietary method, no HPLC