Ultimate innovation

• Buy one Mix2Seq Kit to pay for 96 reactions 
• Share your Mix2Seq data with others via your group
• Transfer Mix2Seq Kits with all rights to someone else
• Name your samples under My Kits & Labels

Highest convenience

• Every Mix2Seq Kit includes 96 barcoded tubes and lids
• Just provide the tubes - no online ordering necessary
• Send us your samples using our free pick-up service
• Use our sample bags and boxes to send your samples

Excellent quality & service

• Data delivery until 12 pm CET next business day with Mix2Seq NightXpress
• Data delivery until 6 pm CET next business day with the regular Mix2Seq service.
• Obtain reading lengths of up to 1100 high quality bases
• Sequencing data are provided in the following file formats by default:
  • .ab1 files - ABI trace files with all raw data
  • .scf files -Trace files with quality values
  • .seq files - Clipped & unclipped FASTA sequences
  • .phd.1 files - With base call and quality information
  • .pdf quality report - Trace file with quality scores
  • FASTA file with all sequences

4 little steps to optimum results

	Your template should consist of 5 µl purified DNA in either of the following concentrations: Plasmid DNA up to 15kbp: 50-100 ng/µl PCR products: 150-300 bp: 1 ng/µl; 300-1000 bp: 5 ng/ µl; 1000 - 3000 bp 10 ng/µl
	Add 5 µl of your SeqPrimer with a concentration of 5 pmol/µl (5 µM).
	Ensure that the total volume of your DNA / primer mix is at least 10 µl.
	Pipet your premixed sample in one of the Mix2Seq tubes and seal the tube with the blue or yellow lid from the enclosed cap pad.

Do not forget to note the barcode number on the side of the tubes before you send us your samples!

Conditions

DNA templates must be purified. Sequencing reactions cannot be repeated and all reactions will be charged. For samples containing difficult-to-read streches (GC-rich, hairpin structurs...) we recommend our TubeSeq Supreme Service.

Please consider optimal primer conditions:

• Primers must not contain phosphorylation or fluorescent dyes
• The optimum primer length is between 16-25 bases
• The primer melting temperature (Tm) should be 50 - 62°C
• The GC content of the primer should be 35-60%
• Ideally one G or C should be located at the 3' primer end
• The number of 3' Gs or Cs should not exceed 2 Gs or Cs
• If possible, avoid >3 identical bases in a row in the sequence

Sample storage:

Premixed samples are not getting stored.

Data storage:

Sequencing data are provided in the following file formats by default and are available online for 6 months:

• .ab1 files - ABI trace files with all available raw data
• .scf files -Trace files with base calls and quality values
• .seq files - Clipped & unclipped FASTA sequences
• .phd.1 files - Contains base call and quality information
• .pdf quality report - Trace file with quality scores
• FASTA file with all sequences

Important information:

Specified delivery times are mainly depending on sample arrival time in the lab.

For many European cities we are providing coordinated pick-up and transport services using Eurofins DropBoxes ensuring on time delivery in the lab.

However, these logistics services may also be subject to force majeure or unpredictable changes caused by e.g. strikes, accidents, storms, floods. In such events, Eurofins Genomics cannot guarantee the stated data delivery time and is not responsible for delays caused by logistics services.

Additionally, our general terms and conditions (GTCs) apply.