siMAX siRNA - The RNA Interfering Molecule

siMAX siRNA - Customised siRNA

siMAX siRNA - Maximum gene silencing with siMAX small interfering RNA

The purpose of siRNAs is to silence several types of RNA. siRNA is a class of double-stranded RNA molecules. It plays an important role in the RNA interference (RNAi) pathway, where it interferes with the expression of specific genes with complementary nucleotide sequence.

Order your Custom siRNA Oligonucleotide

Highlights of our siMAX siRNA

We offer custom siRNAs annealed and ready-to-use:

Guaranteed purity of 85 % (HPLC purified)
Fix quantity of 20 nmol or 40 nmol
Sequence lengths: 17 - 27 bases
Quality control by MALDI-TOF MS
Turnaround time: 8 - 10 working days
Delivery: lyophilised in 2 ml screw cap tubes

Additional online features free of charge:

Selectable label layouts for your oligo tubes
Antisense sequence calculator
Online order status and delivery tracking

A free 1ml 5x siMAX dilution buffer (30 mM HEPES, 100 mM KCl, 1 mM MgCl2, pH = 7.3) is included with each shipment.

21mer versus 27mer siRNA

21mer siRNA molecules are synthesised with either dTdT overhangs or alternate overhangs defined by you:

21mer siRNA

27mer siRNA may show a much higher efficacy than 21mer siRNAs.

27mer siRNA, also known as Dicer-substrate RNAs, is reported to be significantly more potent than the traditional 21mer siRNA by taking advantage of Dicer's natural processing. These dsiRNA molecules can also be chemically synthesized. The structure of these RNA duplexes is shown below:

27mer siRNA

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Related Information

Resuspension & Annealing

To resuspend the siMAX siRNA duplex

Avoid RNA degradation by using special precautions and by wearing protective lab clothing and gloves. All solutions should be qualified as RNAase-free and reserved only for use in RNA experiments. All RNA work spaces must be free of RNAase contamination. Add the required volume by using the provided siRNA dilution buffer to create your stock solution. Buffer must be diluted with RNase-free water prior to use.

Annealing

The delivered siRNAs are annealed and ready to use. But if you want to repeat the annealing step:

Heat the tube to 90°C for 60 - 90 sec
Incubate at 37°C for at least one hour

siMAX siRNA controls

Select the sense strand of your preferred siRNA control for copying it into the siMAX siRNA order page.

siRNA control	Sequence
Lamin A/C µmol	CUGGACUUCCAGAAGAACA
Lamin B2	GAGGAGGAGGAAGCCGAGU
GAPDH	AUUCCAUGGCACCGUCAAG
Luciferase GL2	CGUACGCGGAAUACUUCGA
Luciferase GL3	CUUACGCUGAGUACUUCGA
Green Fluorescent Protein	GGCUACGUCCAGGAGCGCACC
Non Specific Control 31% GC	UAAUGUAUUGGAACGCAUA
Non Specific Control 47% GC	AGGUAGUGUAAUCGCCUUG
Non Specific Control 68% GC	UGCGCUAGGCCUCGGUUGC

Quality is important for us at Eurofins

Our products and services are produced and performed under strict quality management and quality assurance systems.

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