SeqPrimer - Validated to detect single base variants in DNA sequences
Just because primers work in PCR does not mean they provide good sequencing results.
Standard end-point PCR is an exponential process. The primer quality is not crucial for amplification, when checking PCR products on an agarose gel.
In contrast, cycle sequencing reactions require a much higher level of primer quality independent of template type.
Mutations are commonly identified by PCR followed by direct sequencing of the PCR product. The primer quality is therefore even more critical when you want to detect or verify single base variants:
- Genotyping by sequencing
- Microsatellite analysis
- Verification of SDM experiments
That means PCR primers should already have a very good quality when the same primers are used for sequencing the PCR product!
With our validated SeqPrimer, you can be sure to have the optimum primer quality to fulfil this requirement.
The SeqPrimer was developed in close cooperation with our experienced oligo synthesis and DNA sequencing team. It is specified based on a set of analytical quality criteria that were verified in thousands of sequencing reactions.
Standard primers and custom sequencing primers ordered along with DNA sequencing have the same stringent quality criteria.