Cloning Service

Molecular cloning encompasses a range of molecular biology techniques used to assemble recombinant DNA constructs and enable their replication within a suitable host organism.

Eurofins Genomics offers comprehensive cloning services, including TA cloning, cloning of full-length cDNA or PCR products, synthetic gene cloning, and subcloning of vector inserts.

To keep your experience smooth and transparent, every incoming project is initially classified as standard cloning. Once your quote request is submitted, our specialized team performs a thorough review. Should your project fall into the complex cloning category - requiring additional expertise or advanced workflows - we will reach out immediately to discuss options and align with your expectations.

Settings for our standard cloning service are:

• Subcloning of a standard insert (100 – 6,000 bp) or PCR product into a high-copy standard E. coli compatible vector (max. 7000 bp)
• Subcloning via two different sticky end restriction enzymes
• Biosafety level 1

We also offer following molecular cloning techniques on request:

• TOPO Cloning
• Gateway Recombination Cloning
• Gibson Assembly (Isothermal Assembly Reaction)
• Ligation independent cloning

For all quotes, please submit the sequence of your donor and recipient vector. If available, you may also provide the sequence of your final construct to genes.eu@genomics.eurofinseu.com, allowing us to gain deeper insight into your project. If you do not have a final construct yet, our team will design it for you as part of the service.

Please be aware that the following options are not available for mini and midi prep:

• Endotoxin-free preparation
• Sterile preparation
• Animal-free preparation
• Bioburden test
• LAL test

Service description:

• Quality control of vector(s)
• Development of the cloning strategy and subcloning
• Cloning Verification: We analyse up to 6 clones via sequencing of the restriction sites (2 runs)
• Documentation: By quality assurance report, including project specifications, complete plasmid description, restriction map and DNA sequence information
• Provided that there are no unexpected difficulties (biological or technical), the estimated delivery time for the cloning service is approx. 10 business days
• Please note that there can be no guarantee for molecular biology projects
• In case the subcloning fails even after one repetition we would have to charge a set up fee of 30% per subcloning

Confidentiality

It stands to reason that Eurofins Genomics will perform the services outlined above on a “fee for service” basis. This means that Eurofins Genomics has and will claim no rights whatsoever to all or any of the data that will be generated. Eurofins Genomics will take all necessary measures to ensure that none of the data will be disseminated to third parties.

Risk group classification sheet

Please download the risk group classification sheet and send the filled form to genes-eu@genomics.eurofinseu.com after placing your order.