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          Fastest Sanger sequencing of premixed samples in tubes.

          Order Now

           

           

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          A Unique Prepayment Option for our NGS Services

          Learn more

           

           

    • Genotyping & Genexpression
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        • Cancer Ecology
        • Personalised Cancer Therapy
        • Revolutionising human-like-protein production
        • The Microbiome Of Cancer
        • All about biomarker discovery
    • Agrigenomics
      • Markets
      • Agrigenomics
        • Plant Breeder
        • DNA marker discovery
        • Marker-assisted selection
        • GRAS-Di®
        • Microbiome and metagenomics
        • Animal Breeder
        • DNA Marker discovery
        • Pathogen screening
        • Parentage testing
        • Genomic selection
        • Marker-assisted selection
        • Webshop - GenFarmEval
        • Favorite Content
        • Microarrays Accelerate Blue Biotechnology
        • How To Do NGS 50% Faster
        • NGS Portfolio
        • GenFarmEval.com

          Visit our Webshop for Farmers

          learn more

    • Consumer Genomics
      • Markets
      • Consumer Genomics
        • Sequencing Services
        • Genotyping
        • Epigenome Profiling
        • Microbiome Analysis
        • Shotgun Sequencing
        • Whole Genome Sequencing
        • Additional Services
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        • Shipping
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        • The End of Gene Doping
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        • IVDR Compliance
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        • Food Authenticity
        • Meat Traceability
        • Pathogen Traceability
        • Cannabis and Hemp Testing
        • Environmental Testing
        • Non-targeted detection of organisms / species
        • Targeted detection of organisms / species
        • eDNA Tracker
        • Favorite Content
        • Determine the Source of Meat
        • Pine Nuts – Why Testing For Edibility Matters
    • Diagnostic Kit Producer
      • Markets
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        • Synthesis Products
        • Large Scale Oligos
        • Special Requests in Tubes
        • Quality Assurance
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        • The Future of RNA Applications
    • Research / Biotech
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        • Custom DNA Oligos
        • TubeSeq Service Sanger
        • INVIEW Microbiome NGS
        • NGS Services
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        • Mix2Seq NightXpress
        • Express Genes
        • Express GeneStrands
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        • Eurofins Genomics Goes Green
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        • 5 tips to speed up your qPCR
        • Why salt-free oligos are the unsung hero of molecular biology
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siRNA Design Tool

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+49 7531 81 60 68

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support-eu@genomics.eurofinseu.com

Most questions are also answered in our FAQ's.

Questions on your sanger sequencing results?
Click here to contact us

or check our sequencing result guide.

 

   Intro & Info

Eurofins Genomics' siRNA design tool is a proprietary developed software designed to help you selecting the most appropriate siRNA targeting your gene(s) of interest.

The siRNA desgin tool analyses the entered DNA or RNA sequence and chooses the optimum siRNA based on the criterias described by Tuschl et al., Reynolds et al., Ui-Tei et al. and Vert Jp et al. (Please click here for more information)

By calculating the appropriate siRNA sequences, a variety of parameters for the design and the siRNA sequences are already considered and taken as default values. You either can use the default settings or modify those to customise the analysis.

Target Sequence
Copy & paste the target sequence from an external source. DNA or RNA sequence information as well as FASTA sequences ( starting with an ">" followed by the name) are possible. Line breaks and blank spaces are allowed.

Design Parameters
You can design the siRNA from the whole template (= target sequence) or limit the choices to a particular region. The mRNA motifs are the search patterns on the mRNA used to find appropriate siRNA target sites. The input is the DNA coding sequence (cds). you can choose one single motif or a mixture of all motifs (recommended).

Design Exclusion
A-,G-,C- and T- resp. U- stretches (same 3 nucleotides in a raw) are recommended to exclude from the siRNA sequence design. An RNA base at the 3' end of the siRNA sequence is also not recommended. These exclusions are already set as default for the design parameters.

siRNA Parameters
For the siRNA sequences you can define the minum and maximum GC content as well as the distance from start and stop codon.

Click on the "Design siRNA" button to get a list of appropriate siRNA sequences. The best siRNA is most likely the one that shows the highest scores. The selected siRNA sequences can directly be ordered online.

   siRNA Design

Assay Design

siRNA Name

Target Sequence


Design Parameters open / close

Coding Region (whole sequence is default)

From:

To:

mRNA motif: Info

Design Exclusion open / close
siRNA Parameters open / close
 

Minimum

Maximum

GC Content (%):

Distance from start codon:

Distance from stop codon:



* These fields are mandatory
No. siRNA Sequence (Sense) mRNA Motif start c stop c GC Dsir-Score Ui-Tei Score Reynolds Score Order

  • Order
The mRNA motifs are the search patterns on the mRNA used to find appropriate siRNA target sites. The input is the DNA coding sequence (cds). you can choose one single motif or a mixture of all motifs (recommended):

AA(N{19})TT 5-prime-AANNNNNNNNNNNNNNNNNNNTT-3-prime
AA(N{19})NN 5-prime-AANNNNNNNNNNNNNNNNNNNNN-3-prime
NA(N{19})TT 5-prime-AANNNNNNNNNNNNNNNNNNNTT-3-prime
NA(RN{17}Y)NN 5-prime-AANNNNNNNNNNNNNNNNNNNTT-3-prime

IUPAC code:
N= all four bases
R= A or G
Y = C or T/U
Contact Us

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Fri: 8 : 00 AM – 6 : 00 PM, ET

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QUOTES, PRICING & SPECIAL REQUESTS

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Please direct inquires about pricing and special project requests to your sales representative.
General questions: support-eu@genomics.eurofinseu.com

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