The right answers to frequently asked questions
Find the answers to all our products and services by clicking the links below.
What starting material can be accepted?
For a list of accepted starting material, including the relevant amounts, please refer to the Product Specifications.
What kind of quality control do you perform?
NGSelect Amplicon on NovaSeq (150 - 270 bp):
Prior to sequencing, the quality and quantity of each sample will be determined by established methods (e.g. agarose gel analysis / Qubit® Fluorometer / NanoDrop / Agilent 2100 Bioanalyzer). Further quality controls are performed at various steps of the sequencing workflow.
NGSelect Amplicon on MiSeq (280 - 570 bp):
Beside the evaluation of the gel picture you are sending, the success of the PCR reaction serves as our quality control. After the library prep and at various steps of the sequencing workflow further quality controls are performed.
What kind of BioIT do you offer?
Affordable high quality bioinformatics analysis is available for each package (see “Product Specifications” for more.
Can I use the results for diagnostic purposes?
Yes. On request, the service can be performed under diagnostic conditions with ISO17025 certified processes.
Where do I get my results and in which form are they delivered?
The results will be delivered via your Ecom account.
Where should I send my samples?
Eurofins Genomics Europe Pharma and Diagnostics Products & Services Sanger/PCR GmbH
Jakob-Stadler-Platz 7
78467 Konstanz
Germany
For the product NGSelect Amplicon on MiSeq, can we send samples that consist of several amplicons generated with different primer pairs?
Usually you would send per sample 1 amplicon generated with 1 primer pair. In some cases you might require however less reads per amplicon as given in the Project Specification. In such cases the individual amplicon samples you send may also consist of several amplicons. Please note, that in this case however we will not perform the clipping of primer sequences and deliver raw data only.
How should I send my samples?
- Please use 1.5 ml safe-lock tubes for your templates and primers
- Do not tape or wrap tubes with parafilm. Safe-lock tubes offer perfect sealing and evaporation protection
- Label your template with our free NGS barcode labels.
If you send more than 92 samples then you can also sent the samples in plates. Please use full skirted PCR plates (e.g. Eppendorf or Twin).
For more information and for sample submission for extraction please read our Sample Submission Guide.
(Picture shows the procedure with Sanger Prepaid Barcodes but work the same with free NGS Barcodes)
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Only use 1.5 ml safe-lock tubes
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Remove barcode stricker from film
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Affix barcode sticker horizontally
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QR-code visible at front so it can be read by scaner
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Does Eurofins Genomics perform a QC on my samples before library preparation?
Yes, we do perform a QC of the quantity of the amplicons.
What are TruSeq adapter sequences?
The adapter sequences are:
Forward: 5’-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’
Reverse: 5’-GACTGGAGTTCAGACGTGTGCTCTTCCGATCT-3’
I have mixed amplicons in a single sample that I want to sequence. Can I get the unique sequences from this mixture, and how can your service help with this?
Yes, you can send in a single sample containing mixed amplicons for sequencing. Our Unique Sequence Analysis service is specifically designed to handle such samples. By using advanced sequencing and bioinformatics techniques, we can accurately separate and identify each unique sequence, ensuring you get comprehensive and reliable results from your mixed amplicon sample.
How can I order my needed service?
Please go to our website https://eurofinsgenomics.eu/, select your wished service and follow the instructions. Depending on the selected product you might need to raise a quote first. Once your quote is available you will be informed, and you can accept it in your account (please navigate to “Account” -> “Quotes”).
During the ordering process you will be asked to upload your samples via our Sample Submission Sheet. You can find the option to download the sheet directly next to the upload button (please always download the sheet here as it can differ between the products we offer). This sheet requires you to give us more information about your samples, as well as assigning Eurofins NGS Barcode Labels to your samples.