For 150-270 bp amplicons sequenced on our NovaSeq, please use our Inline Tag Design Tool : NGS Adapter Ligation Oligos (eurofinsgenomics.eu). When ordering the service, select the optional service “Tag sorting – Eurofins Genomics Design”. You will receive sorted sequences (FASTQ).

• 192 individual inline tags are available, each consisting of 10 nucleotides added to the forward primer.
• There is a minimum edit distance of 4 to prevent misassignments due to sequencing errors.
• The tags are optimized for both low and high sample pools.
• All NGSgrade Oligos undergo specific processes to ensure cross-contamination-free and high-purity performance.
• For multiplexing 24 samples with the same target sequence, 24 forward primers (each with a unique inline tag) and one reverse primer (without tags) are required.