The right answers to frequently asked questions
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General Questions regarding Sample Submission, Ordering & Sample Shipment & Results can be found here:
How should I purify my DNA?
We recommend purifying your DNA with commercially available kits based on DNA-binding beads or columns.
How do you calculate coverage?
Coverage is calculated using the formula:
(Read length) × (Total number of reads) ÷ (Genome size).
For example, sequencing a genome of 10 Mb with 20 million reads at 2 × 150 bp read length results in a coverage of 60×. "
How much should I sequence?
Depending on your needs we recommend the following coverage:
- Germline/frequent variant analysis: 20-50x
- Somatic/rare variants: 100-1000x
- De novo assembly: 100-1000x