Here we summarised all imporant information that you might need to know, when planning to order Gene Fragments.
Optimisation (optional)
Sequences of high complexity may prolong the overall turnaround time of delivery and in some extreme cases disqualify the sequence for delivery as a GeneStrand. Luckily, Eurofins Genomics offers free optional sequence optimisation with its GeneStrands service, which has been shown to alleviate complexity over 90% of the time.
Try Our Free GENEius Optimisation Tool
Note: In order to optimise a given GeneStrands sequence, an ORF needs to be present.
If you would like us to optimise your GeneStrands please send your sequence to genes-eu[at]eurofins.com
Quality Assurance
All GeneStrands are synthesised under the same meticulous conditions as our gene synthesis service. However, GeneStrands are delivered as uncloned products, and as such the presence of minute populations of truncated or mutated species is unavoidable.
All GeneStrands are assembled from high-quality oligonucleotides synthesised at our in-house DNA synthesis facility. Assembled fragments are then size confirmed via electrophoresis.
Sequence-verified GeneStrands undergo DNA sequencing at our in-house sequencing facility. Sequence validation is of the entire pool, so the results obtained is a representation of the predominant sequence within the pool. Sequence verification is only possible for fragments ≥150 bp.
Eurofins Genomics routinely tests the mutation rate and quality of its synthesised Gene Fragments, and we have observed over 85% reliability of obtaining a correct clone if 6 or more colonies are picked and sequenced.
Delivery and Documentation
The synthetic linear dsDNA will be delivered as a fragment. If you order your Express GeneStrands until 2 p.m. CET we will ship them the next working day. If we cannot keep the next day shipment, we deduct the Express Fee from your invoice.
48h delivery available in all European countries and regions where UPS is offering Express shipment. Please check for your area: https://wwwapps.ups.com/ctc/request?loc=en_DE
If sequence validation is choosen: Electronic DNA sequencing data files will be delivered via Email.
We ensure strict confidentiality of every project. The high quality standard of our service is permanently assured by a professional quality management.