Molecular biology service: Research through expertise

IVT mRNA synthesis

In Vitro Transcribed (IVT) mRNA presents a versatile and cost-efficient solution for generating mRNA transcripts, suitable for a wide array of applications including therapeutic and vaccine development, gene editing, gene expression investigations, antibody creation, and more. IVT mRNA synthesis can be initiated from circular plasmid DNA, PCR products, and double-stranded oligonucleotides featuring promoter sequences derived from phage RNA polymerases, with T7 being the most prevalent.

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Overview

Eurofins Genomics provides a comprehensive range of services, encompassing diverse DNA template options to facilitate IVT mRNA production. All our mRNA offerings are equipped with cap structures and polyadenylation tails to guarantee stability and optimal performance in subsequent applications.

Our standard quality control procedures encompass evaluations of size, purity, and integrity. For an added layer of quality assessment, methods like capping efficiency measurements are also accessible.

Product description

Template synthesis & preparation, including plasmid DNA, PCR products and synthetic oligonucleotides
Codon optimisation, various capping and tailing strategies for enhancing mRNA stability and translation
Incorporating modified nucleotides during synthesis to increase protein expression and decrease innate immune response

Specifications of mRNA and quality control

QC Item	Test Method	Specification	Standard	Upgrade
Appearance	Visual inspect	Clear, no visible particles	Yes	Yes
pH	pH meter	Target ± 0.5	Yes	Yes
Concentration	Nanodrop	0.5-1mg/ml	Yes	Yes
Purity	Nanodrop	260/280 is 1.8-2.3, 230/280 ratio is 2.0-2.3	Yes	Yes
RNA Length and Integrity	Gel electrophoresis	Expected fragment size observed	Yes	Yes
RNA Length and Integrity	Capillary electrophoresis	DV200 metric evaluation	No	Yes
Capping Efficiency	Enzyme digestion and gel electrophoresis	≥ 90%	No	Yes
Endotoxin	Kinetic turbidimetric assay (KTA) - limulus amebocyte lysate (LAL)	< 10EU/mg	No	Yes

How to order IVT mRNA

You can order our mRNA via the gene synthesis project page. Please upload the PDF file with all your details and upload it on the gene synthesis project page.

A quote will be prepared and send to your personal my quotes page.