MYCOPLASMACHECK TUBE | BARCODE

Mycoplasmacheck Service -
Mycoplasma testing in every cell line

Easy outsourcing of mycoplasma testing in any cell line

Mycoplasmacheck is a qPCR-based service for detection of mycoplasma in cell cultures.

Dear customer, if you send your samples via our dropboxes, please make sure to order the orange stickers below directly with your barcodes. This will help to reduce our carbon footprint. Thank you very much.

Prepaid MPC Barcode

Mycoplasmacheck Barcodes

Order your barcodes in advance
Label your samples and benefit from secure sample assignment
No order needs to be placed when sending the samples

Product Code: 50400400

Mycoplasmacheck barcodes remain valid for five years from the date of ordering, after which they expire.

Want to use our Dropboxes to send your samples?

Please order the stickers below and put them on the bag / box where your samples are shipped in (within the envelope). This will ensure that your samples are routed savely and fast to our mycoplasmacheck testing laboratory.

Please find your closest dropbox location here >>

Sticker to send your MPC samples via our Dropboxes

Convenient sample shipment.

One unit includes 10 shipment stickers
You only need one sticker per shipment

Why should I test my cell lines for Mycoplasma contamination?

As contamination of mycoplasma alters cell-line properties, their unnoticed presence often leads to non-reproducible results. Regular testing with Mycoplasmacheck serves as a rapid, accurate and objective means of checking cells for mycoplasma contamination, ensuring cell lines are kept reliably clean.

Highlights of the Mycoplasmacheck Service:

Standardised qPCR test for mycoplasma
Data provided online within 48 hours after sample receipt
Test history report for select periods of time can be compiled online at any time

>> FAQ's MPC

>> MPC Results

Product details & sample submission

Product Details

Details of our Mycoplasmacheck

Full-service package offered by Mycoplasmacheck:

Detection of all mycoplasma species listed in the European Pharmacopoeia 2.6.7: M. arginini, M. fermentans, M. orale, M. hyorhinis, M. hominis, M. genitalium, M. salivarium, M. synoviae, M. pirum, M. gallisepticum, M. pneumoniae, M. yeatsii, Spiroplasma citri and Acholeplasma laidlawii
Identification of more than 100 additional mollicutes
Highly sensitive assay capable of detecting 1 international unit (IU) per PCR of the World Health Organization’s (WHO) international standard for mycoplasma DNA (Please see application note)
Highly specific test that identifies ten copies of mycoplasma per reaction with no cross-reactivity to E. coli or eukaryotic DNA
Use of positive and negative controls to monitor test performance
Exclusively licensed from the German Cancer Research Center, (Deutsches Krebsforschungszentrum, DKFZ), Heidelberg, Germany
Easy sample drop-off at any Collection Point or ship separately

Technology used for Mycoplasmacheck

Roche LightCycler 480 II

Deliverables of Mycoplasmacheck

Data report indicating mycoplasma status, as well as any PCR inhibitors found in the sample
The user can compile a PDF document online at any time to view the results from any specific testing period.
PDF document serving as an independent certificate of results; suitable for direct submission to journals or granting agencies

Additional Information

Reliably detect all common mycoplasma species

Mycoplasma contamination adversely affects 35% of the world’s cell lines.

As signs of mycoplasma are easy to miss, only regular monitoring of cell cultures can ensure that research results are valid and reproducible. With its standardised workflows, we can rapidly and reliably detect all common mycoplasma species.

Table 1. List of mycoplasma species frequently detected in cell cultures. These mycoplasma, all found in the European Pharmacopoeia 2.6.7., are readily detectable with Mycoplasmacheck.

As a qPCR-based assay, the technique is more accurate and sensitive than traditional microbial culture methods and bioluminescence-based methods for mycoplasma detection (Fig1). Besides severely reducing the hands-on time involved in in-house testing, outsourcing the task of mycoplasma detection offers distinct benefits that cannot be matched by do-it-yourself kits (Fig 2).

Fig. 1. Comparison of three different methods for detection of mycoplasma in cell cultures. The graph demonstrates the superiority of mycoplasma PCR over culture-based or bioluminescence-based (Mycoalert) techniques in analysing cell lines for mycoplasma (adapted from Cytotechnology (2014) 66:861–873)

Fig. 2. Advantages of using Mycoplasmacheck for mycoplasma PCR detection over in-house PCR kits

Publications

Here you can find a selection of publications from our customers, that tested their cell lines for mycoplasma contamination

2024

Mycoplasmacheck: Pierre Joanne, Yeranuhi Hovhannisyan, Alexandre Simon, Gaëlle Revet, Romain Diot, Gabriel Friob, Denisa Calin, Zhenlin Li, Anthony Béhin, Karim Wahbi, Gérard Tachdjian, Onnik Agbulut, Generation of human induced pluripotent stem cell lines from five patients with Myofibrillar myopathy carrying different heterozygous mutations in the DES gene, Stem Cell Research

Mycoplasmacheck: Marta Ribeiro, Sandra Martins, Teresa Carvalho, Marta Furtado, Joaquim Sampaio Cabral, Dulce Brito, Maria Carmo-Fonseca, Simão Teixeira da Rocha,Generation of induced pluripotent stem cell lines from two unrelated individuals with familial hypertrophic cardiomyopathy carrying the MYBPC3 missense c.1484G>A mutation, Stem Cell Research

Mycoplasmacheck: Laetitia Duboscq-Bidot, Bénédicte Hoareau, Flavie Ader, Vincent Fontaine, Eric Villard, Generation of CRISPR-Cas9 edited human induced pluripotent stem cell line carrying BAG3 V468M mutation in its BAG domain, Stem Cell Research

Mycoplasmacheck: Ujevic, A., Knizkova, D., Synackova, A. et al. TBK1-associated adapters TANK and AZI2 protect mice against TNF-induced cell death and severe autoinflammatory diseases. Nat Commun

Mycoplasmacheck: Jacquemin, C., El Orch, W., Diaz, O. et al. Pharmacological induction of the hypoxia response pathway in Huh7 hepatoma cells limits proliferation but increases resilience under metabolic stress. Cell. Mol. Life Sci.

Mycoplasmacheck: Derouane, F., Desgres, M., Moroni, C. et al. Metabolic adaptation towards glycolysis supports resistance to neoadjuvant chemotherapy in early triple negative breast cancers. Breast Cancer Res

2023

Mycoplasmacheck: Abouzayed A, Zedan W, Altai M, Strand J, Örbom A. Co-injection of anti-HER2 antibody Trastuzumab does not increase efficacy of [¹⁷⁷Lu]Lu-PSMA-617 therapy in an animal model of prostate cancer. Am J Nucl Med Mol Imaging.

Mycoplasmacheck: Marianne Burbage et al. ,Epigenetically controlled tumor antigens derived from splice junctions between exons and transposable elements.Sci. Immunol.

Mycoplasmacheck: Sarah Ceylan Krassnig, Marina Mäser, Nicola Anna Probst, Jens Werner, Charlotte Schlett, Nina Schumann, Gudrun von Scheven, Aswin Mangerich, Alexander Bürkle, Comparative analysis of chlorambucil-induced DNA lesion formation and repair in a spectrum of different human cell systems, Toxicology Reports

Mycoplasmacheck: Pandiloski, N., Horváth, V., Karlsson, O. et al. DNA methylation governs the sensitivity of repeats to restriction by the HUSH-MORC2 corepressor. Nat Commun

2022

Mycoplasmacheck: Gizon et. al., Generation of a heterozygous SCN5A knockout human induced pluripotent stem cell line by CRISPR/Cas9 edition

2021

Mycoplasmacheck: Kristiansson et. al., 177Lu-PSMA-617 Therapy in Mice, with or without the Antioxidant α1-Microglobulin (A1M), Including Kidney Damage Assessment Using 99mTc-MAG3 Imaging

Mycoplasmacheck: Goisnard et. al., The New Serum-Free OptiPASS® Medium in Cold and Oxygen-Free Conditions: An Innovative Conservation Method for the Preservation of MDA-MB-231 Triple Negative Breast Cancer Spheroids

Mycoplasmacheck: Sacchi et. al., Nanogold Functionalized With Lipoamide-isoDGR: A Simple, Robust and Versatile Nanosystem for αvβ3-Integrin Targeting

Mycoplasmacheck: Malakpour-Permlid et. al., Identification of extracellular matrix proteins secreted by human dermal fibroblasts cultured in 3D electrospun scaffolds

Mycoplasmacheck: Groisnard et. al., LightSpot®-FL-1 Fluorescent Probe: An Innovative Tool for Cancer Drug Resistance Analysis by Direct Detection and Quantification of the P-glycoprotein (P-gp) on Monolayer Culture and Spheroid Triple Negative Breast Cancer Models

Mycoplasmacheck: Boehm et. al., SMG5-SMG7 authorize nonsense-mediated mRNA decay by enabling SMG6 endonucleolytic activity

Mycoplasmacheck: Perrin-Cocon et. al., A hexokinase isoenzyme switch in human liver cancer cells promotes lipogenesis and enhances innate immunity

Mycoplasmacheck: Plavec et. al., Targeting of fluorescent Lactococcus lactis to colorectal cancer cells through surface display of tumour-antigen binding proteins

2020

Mycoplasmacheck: Janz et. al., Generation of two patient-derived iPSC lines from siblings (LIBUCi001-A and LIBUCi002-A) and a genetically modified iPSC line (JMUi001-A-1) to mimic dilated cardiomyopathy with ataxia (DCMA) caused by a homozygous DNAJC19 mutation

Mycoplasmacheck: Nesteruk et. al., Forced expression of HOXA13 confers oncogenic hallmarks to esophageal keratinocytes

Mycoplasmacheck: Gretarsson et. al., Dppa2 and Dppa4 counteract de novo methylation to establish a permissive epigenome for development

Mycoplasmacheck: Tesauro et. al., Different Camptothecin Sensitivities in Subpopulations of Colon Cancer Cells Correlate with Expression of Different Phospho-Isoforms of Topoisomerase I with Different Activities

Mycoplasmacheck: Chua et. al., The NALCN channel complex is voltage sensitive and directly modulated by extracellular calcium

Sample preparation

Pre-requisites prior testing

For maximum sensitivity: Recommendations for cell culture cultivation prior to testing:

Cell lines should be cultured in the absence of mycoplasma-active antibiotics for one week prior to submission. Penicillin and streptomycin do not affect mycoplasma nor do they inhibit MYCOPLASMACHECK.
Cell cultures should be maintained in the same media without dilution with fresh media for three days before testing.
Cryopreserved cell cultures should be cultured about two weeks prior to sample submission.
80-90% confluence of the tissue culture is recommended for testing. Inhibiting substances may accumulate in >90% confluent cultures.

Sample preparation

Sample preparation of cell cultures:

Transfer 500 μl of cell culture supernatant from the test cell culture to a 1.5 ml tube. The lid should be sealed tightly to prevent opening during heating.
For suspension cell lines, stand culture flasks vertically allowing cells to settle for about 30 minutes prior to removal of 500μL of the supernatant and transfer to a 1.5 ml tube.
Boil supernatant at 95 °C for 10 minutes.
Briefly centrifuge (5 seconds) the sample at ~13,000 rpm to pellet cellular debris.
Transfer 100 – 200 μl of supernatant into a new 1.5 ml tube labelled with a MYCOPLASMACHECK barcode. Do not disturb pellet

Sample preparation of cell cultures that are prone to PCR inhibition:

For adherent cell lines:

Transfer 500 μl of cell culture supernatant from the cell culture to a 1.5 ml tube.
Centrifuge sample for 10 min at >10.000 x g to pellet mycoplasma cells.
Discard 450 μL of supernatant without disturbing any pellet.
Boil remaining 50μL at 95 °C for 10 minutes. The lid should be sealed tightly to prevent
opening during heating.
Perform DNA extraction using any commercially available DNA extraction kits according to
the manufacturer ́s instructions.
Elute in 30 μL using a 1.5 ml tube labelled with a MYCOPLASMACHECK barcode.

For suspension cell lines:

Centrifuge 500 μL sample for 3 min at >300 x g to pellet eucaryotic cells.
Transfer supernatant in new 1.5 ml tube.
Continue with 2nd step above.

Sending samples

Sending samples according to the requirements below helps us to do our job better
and provides you with accurate results.

Shipping address (if no dropbox is used):

Eurofins Genomics Europe Food/Environment/White Biotech Products & Services GmbH
FEB species determination
Anzinger Strasse 7a
85560 Ebersberg

Sample submission

Use 1.5 ml safe-lock tubes for your templates and primers
Do not tape or wrap tubes with parafilm. Safe-lock tubes offer perfect sealing and evaporation protection
Label your template with our Mycoplasmacheck barcode labels.
Use a water resistant marker for any additional labeling of your tubes

This picture helps you to stick the labels correctly to the tube. Please note, these are NOT MPC barcodes. But still it should be handled the same way.

Only use 1.5 ml
safe-lock tubes

Remove barcode
stricker from film

Affix barcode sticker
horizontally

QR-code visible at front so
it can be read by scaner

Order Management

Mycoplasmacheck (MPC) Barcodes

The Barcodes can be found in the "Barcode Management Genotyping" list

Order updates

When your samples arrive in our lab an Email will be sent to you and an order will be visible under my orders
When samples are finished you will receive a "Partially complete" or a "Complete" Email
- Partially complete = not all samples are finished
- Complete = all samples are finished
The results are available in the Mycoplasmacheck results
- You can easily create a report for one or more samples
- Share your results with your colleagues
- Sort and filter according to the results and date range