Fish Determination and Authenticity Testing.

6SEQ-000BJ014

All fish species

No mixtures allowed - only pure material

Qualitative determination of fish species.
Please provide information about expected species if present. 

6SEQ-000BJ020

Species identification crustacean

No mixtures allowed - only pure material

Identification of subspecies or species of crustacean (shrimp / prawn, crabs, lobsters) in fish samples.
Please provide information about expected species if present. 

 6SEQ-000BJ120

Species identification squid / octopus

No mixtures allowed - only pure material

Identification of subspecies or species of squids/octopus in fish samples.
Please provide information about expected species if present. 

 6SEQ-000BJ220

Species identification Mussel (Scallops & Mytilus)

No mixtures allowed - only pure material

Identification of species of mussels (Mytilus and Scallops only) in mussel samples. For speciation of other mussel species please contact us. For Mytilus: Mytilus edulis and Mytilus chilensis and hybrids of both as well as Mytilus galloprovincialis can be clearly distinguished. For Scallops: Pectinidae genera like Pecten, Argopecten, Aequipecten, Plagopecten etc. but also Chlamys and Zygochlamys etc. are included.
Please provide information about expected species if present. 

6PCR-000BJ019

Species determination Tuna

Identification of tuna species in a fresh/frozen fish sample, salad (but too much vinegar can hamper DNA analytic) or on (frozen) pizza (all tuna species possible).

6PCR-000BJTUN

Canned tuna authenticity

Differentiation between Katsuwonus pelamis and Thunnus spp. This test is highly recommended for canned tuna samples where DNA quality and quantity is low due to sample processing.

6PCR-000BJF11

Fish species detection (qualitative) 11 species

The test is applied on fish and fish products, e.g. fish burger and fingers, as well as on processed food, e.g. ready meals, protein powder, trace samples and feed, to detect the animal source it was produced from.

Real-time PCR assays highly specific for the species Norway and Atlantic Pollock (Theragra chalcogramma, Theragra finnmarchica; taxonomically the same species), Salmon (Salmo salar), Shark Catfish (Pangasianodon hypophthalmus), Pink Salmon (Oncorhynchus gorbuscha), Coho Salmon (Oncorhynchus kisutch),  Rainbow Trout (Oncorhynchus mykiss), Haddock (Melanogrammus aeglefinus), Pacific Cod (Gadus macrocephalus), Atlantic Cod (Gadus morhua) and Saithe (Pollachius virens) are applied to detect the presence of these animal species.

6CE0-000BJ034

Fish authenticity using NGS

Metabarcoding analysis of food and feed samples by Next Generation Sequencing to detect all fishes present in a sample without necessary prior knowledge about expected species.

This approach is suitable for complex mixtures of different species (> 2 up to unlimited), e.g. present in fish meals / fish balls / feed / other complex fish mixtures, etc.

This service includes:

• DNA extraction from complex fish products
• One quality check per DNA sample by quantitative real-time PCR (Mito-PCR animal DNA)
• Generation of 2 amplicons per DNA sample (CytB, 16S),  + H₂O control
• Sequencing of the amplicons on MiSeq
• Basic bioinformatical analysis (sorting of reads according to inline barcodes)
• Advanced bioinformatical analysis for taxonomical read assignment and species determination
• Individual reporting

PLEASE NOTE:Sample processing starts only on Monday of samples that arrived Friday the week before the latest.