INVIEW Microbiome 16S-Full-Length

Amplicon Metabarcoding

Species identification

Metabarcoding is a DNA-based technique used to identify and analyze multiple species in a sample by sequencing specific genetic markers, known as barcodes. These barcodes are unique to each species, allowing for the detection of a wide range of organisms, including bacteria, fungi, plants, and animals, from environmental samples like soil, water, or air. The sequences are then compared to reference databases to provide a detailed profile of the species present, offering insights into biodiversity and ecosystem health.

Our metabarcoding service offers precise and highly sensitive identification and classification of a wide range of organisms. The service covers DNA amplification, library preparation, sequencing on Illumina's MiSeq platform, and advanced bioinformatics to deliver detailed insights into food safety, biodiversity and ecosystem health.

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Service

We provide optional bioinformatics such as metabarcoding profiling.

Optional DNA isolation service: Please refer to our DNA isolation guide. Please note that we do not accept samples with higher biosafety level than S2. GMOs are only accepted with S1 level.

Highlights

We use specialized genetic markers for:

16S for bacteria
cytB and COI for fish & seafood
Mitochondrial 16S for land vertebrates
18S and ITS regions for fungi
trnL and ITS for plant identification

Next-generation sequencing for the identification of all species of the above categories in mixed samples

Guaranteed 60,000 reads for enhanced data quality
Services conducted in ISO 17025 certified laboratories
Comprehensive bioinformatics services
Data transfer via secure FT

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Product Specifications & Ordering

Specifications

Generation of 1 – 4 amplicons per DNA sample (you can choose from our target list)
Quality control assessment after target specific amplification
Pooling & normalisation of amplicons
Sequencing on Illumina´s MiSeq with the 2 x 300 bp paired-end read module
Delivery of guaranteed 60,000 read pairs per amplicon (+/- 3%)

Starting material

Sample Type: Purified DNA
Purity (OD260/280): 1.8-2.0
Volume: 1 target: 20µL for each additional target plus 10 µL (e.g. 50 µL for 4 targets)
Concentration: 10-50 ng/µL
Minimum Amount: 200 ng
Resuspension Buffer Water, EB, or low TE (<0.1 mM EDTA)
Format: barcode labelled 1.5 ml safe-lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, semi-skirted, leave position G12 & H12 empty
Shipment Method: Room temperature/ice

Deliverables

Raw data FASTQ files
Optional analysis for comprehensive metabarcoding profiling:
- Comprehensive profiling report (.PDF)
- Taxonomy tables (.TXT)
- OTU tables, including normalized abundance estimation (.TXT, .BIOM)
- Read sequences of OTU representatives (.FASTA)
- Processed reads sequences that went into the OTU picking process (.FASTA)
- Taxonomy plots