Custom long-read amplicons

Custom Long-Read Amplicon Sequencing

Custom Long-Read Amplicon Sequencing using Oxford Nanopore Technology.

Long-read amplicon sequencing is a uniquely designed service for scientists looking to perform full-length sequencing and annotation of complex amplicons between 500 bp - 25 kb in length using Oxford Nanopore Technologies.

A complex amplicon is a PCR product characterized by high genetic variability, structural variations, or mixed populations, such as those found in 16S rRNA studies, making sequencing and analysis more challenging.

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Applications & services

16S Microbiome Analysis
Unique Sequence Analysis
Consensus Sequence Analysis
Variant Analysis - Germline
Sequence Cleaning
Host Removal
Pod5 raw data delivery

Specifications

Not suited for ssDNA
Ligation-based library preparation (amplification-free) using v14 library prep chemistry
Up to 96 samples per flow cell
Sequencing using Oxford Nanopore GridION (flow cell R10.4.1)
Custom Long Read Amplicon Sequencing dedicates an entire flow cell to your amplicons
Output read length: Equal to fragment length
Output is dependent upon the number and length of the amplicons, approx. 50.000 reads per amplicon typically obtained

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Starting material, deliverables, & sample submission

Deliverables

Raw Nanopore amplicon data (FASTQ)
Raw Nanopore sequencing data (POD5) available on request (additional costs)
Customizable data analysis packages are available in our ordering form, e.g. Variant analysis VCF-file