ONT Lite Bacterial Genomes

ONT Lite Bacterial Genome Sequencing

Bacterial genome sequencing using Oxford Nanopore Technology

Tailored for the complete sequencing and single contig assembly of genomic DNA (gDNA) from an individual, potentially unknown bacterial strain, our bacterial genome sequencing service employs state-of-the-art Oxford Nanopore Technologies (ONT) long-read sequencing technology.

Take advantage of our super low prices and exceptionally fast turnaround time.

Specification

Sequencing on Oxford Nanopore GridION
On average 30x genome coverage
De novo assembly and QC, species identification, and annotation included in the package
Prepaid option available
From one sample
Great price, fast turnaround time
For all bacterial genomes up to 7 Mb

Applications

De novo assembly of bacteria (< 7 Mb) and intrinsic plasmids (no reference sequence required)
Sequencing of a clonal population (individual strain, potentially unknown)
Species determination
Sample clonal/purity check after the assembly

Need more?

Your bacterial genomes are larger?
You need more coverage?
You need a hybrid assembly?

Learn more on our "Complete ONT portfolio overview">>

>> FAQ's Bact. wGS

>> Trouble- shooting

>> how to redeem prepaid coupons

>> Sequencing Accessories

Product Specifications & Ordering

Product details / workflow

No sample number limitation, dropbox shipment possible 
Super fast turn-around time in 5-7 working days
Prepaid option available
On average 30x genome coverage
De novo assembly and QC, species identification, and annotation included in the package
Library preparation (amplification-free, including minimal fragmentation); using v14 library prep chemistry
Sequencing using Oxford Nanopore GridION (flow cell R10.4.1)

Data delivery & Bioinformatics deliverables

The following files will be delivered for each bacterial genome sample

Comprehensive data analysis report with assembly statistics and QC in HTML format
FASTQ raw data delivered which align to the consensus sequence
Assembled genome sequence in FASTA format
Circular genome plot as a publication-ready PNG
Annotated genome sequence of all contigs in GENBANK format (import directly into your favourite software like Sanger's Artemis, SnapGene, Geneious Prime, Benchling, SeqBuilder Pro etc.)
Annotated genome features in GFF3 format
Annotated feature table, including the nucleotide sequence of each feature
Nucleotide sequences of all gene sequences in FASTA format
Amino acid sequences of all annotated protein sequences in FASTA format

Your data will be securely delivered through your Ecom account

No delivery of: Pod5 or FASTQ files that don't align.

If you are interested in Pod5 files and not-aligned FASTQ files please contact us, regarding our premium service.

Delivery time

The turnaround time for bacterial whole genome sequencing is on average 5 - 7 business days.

You will be informed via Email, when your results are available in your Ecom account.

Sample Preparation & Submission

Use the following concentration and volume for your samples:

Sample type	Length	Concentration	Min. Volume
Bacterial genome	up to 7 Mb	30 ng/µl	20 µl

Please note: Samples that do not meet our concentration requirement might fail in sequencing. No repetition will be performed on failed samples due to wrong conc adjustment.

For DNA sample preparation it is sufficient to use a high quality column based DNA preparation kit. No high molecular weight DNA is required.

Please send your sample tubes according to the requirements below:

Use 1.5 ml safe-lock tubes for your samples
Please provide your samples either in nuclease-free water or elution buffer (10 mM Tris, pH 8.5)
Please do not use Nanodrop for your concentration measurement. Spectrophotometric techniques are unreliable for the quantification measurement. Please use a fluormetric method, like Qubit.
Do not tape or wrap tubes with parafilm. Safe-lock tubes offer perfect sealing and evaporation protection
Label your sample tubes with our free barcode labels. Affix the label horizontally

How and where to ship your samples:

Use either one of your nearest DropBox for free sample pick up or a courier of your choice
You can convinently use one of our sample shipment options to send your samples
To send your samples to us safely, make use of our sample bags and boxes
Send your samples to our Eurofins Genomics sequencing lab in Cologne, Germany

Please ensure that your sample is free of any contaminants (e.g., ethanol) that could negatively affect your results. A complete list of contaminants can be found here: https://community.nanoporetech.com/contaminants

Literature

Demo report for bact. WGS