INVIEW Oncoprofiling (728 genes)

INVIEW Oncoprofiling - 728

Validated cancer panel for analysis of 728 key cancer-specific genes from tissue biopsy samples and genomic DNA

INVIEW Oncoprofiling is among the most comprehensive gene panels for optimized detection of tumor-specific genomic alterations, delivering accurate genomic insights from FFPE, cfDNA, fresh-frozen tissue, and genomic DNA samples. Covering the full exons of approximately 728 well-characterized cancer genes—including tumor activators, suppressors, and drug resistance biomarkers—this solid tumor panel enables reliable detection of clinically relevant driver mutations. It also supports tumor mutational burden (TMB) analysis for predicting immune checkpoint inhibitor therapy response using tumor-only samples, eliminating the need for matched normal controls.

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Highlights

Highly uniform coverage of all exons in 728 cancer-associated genes
Detection of relevant single nucleotide variations (SNVs) and insertions and deletions (InDels), structural rearrangements and copy number variations (CNV) in genes, selected promotor regions, miRNAs, and extra-exonic variants
Determination of tumour mutational burden (TMB) status of the patient
Ultra-sensitive detection levels down to 1% allele frequency
Proprietary protocols for low-input DNA library preparation from challenging tissue and FFPE samples as well as cfDNA (INVIEW Liquid Biopsy Oncoprofiling)
Advanced bioinformatics services with detailed, customisable analysis reports
Data transfer via secure FTP

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Product Overview


Parameter	INVIEW Oncoprofiling 728	INVIEW Liquid Biopsy Oncoprofiling 728
Starting material	gDNA, FFPE DNA	cfDNA
Batch size	From 1 sample	From 1 sample
On-target coverage	Approx. ~500x	Approx. ~500x
Flexible coverage available	Yes	Yes
Analysis of somatic mutations	+++	+++
Analysis of germline mutations	+++	+++
Delivery time*	20 days up to 50 samples	30 days up to 32 samples
Express delivery time*	14 days up to 16 samples	14 days up to 16 samples

*For high-throughput projects, please inquire for custom options.

Specifications

QC of DNA for both quantity and integrity
Illumina-compatible DNA library preparation using Unique Dual Indexing (UDI)
Library indexing offered at post-capture stages of the hybridization workflow using Eurofins´ proprietary INVIEW Oncoprofiling 728 panel based on Agilent SureSelect technology
- 728 protein-coding genes, selected promoter regions
- 81 miRNAs
- 5 promoter regions
- 281 extra-exonic variants (introns, regulatory regions, untranslated regions (UTRs) and intergenic regions)
Technology: Illumina NovaSeq
Run type: paired-end
Read length: 2 x 150 bp
Guaranteed 15 Mio read pairs (~ average 500x on-target coverage) plus flexible addon read packages as multiples of 5 Mio read pairs
Depending on your needs we recommend the following coverage:
- Somatic/rare variants: 100x

Starting material

Sample Type: Genomic DNA (RNA-free)
Minimum Amount: 100 ng (gDNA), 200 ng (FFPE DNA), 30 ng (cfDNA)
Volume: 20 to 100 µL (gDNA + FFPE DNA), 20 to 30 µL (cfDNA)
Concentration: >1 ng/µl (gDNA, cfDNA), >2 ng/µl (FFPE DNA)
Purity (OD260/280): 1.8-2.0
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Format: barcode labelled 1.5 ml Eppendorf Safe-Lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, full-skirted, leave position G12 & H12 empty 
Shipment Method: room temperature

Applications & services

Our Variant Analysis service includes detection and annotation of germline single nucleotide polymorphism (SNPs), insertions and deletions (InDel).
Additionally, we provide optional services such as tumour mutational burden (TMB), microsatellite instability (MSI) status, structural variant analysis, copy number variation detection, tumor vs. normal and tumor-only (somatic) variant analysis to pinpoint genetic mutations in tumor samples, joint-genotyping to analyze multiple samples for shared genetic variations, and population genotyping to understand genetic diversity within specific groups.

CNV analysis should be performed in paired analysis of tumour versus matched normal tissue which is used as reference.
In the case of tumour FFPE samples, we strongly recommend sending a healthy tissue FFPE sample from the same patient. Alternatively, tumour FFPE samples from at least seven other patients can be sent.
For fresh frozen tissue, we strongly advise that blood or healthy tissue from the same patient is sent. Alternatively, fresh frozen tissue from at least seven other patients or controls is recommended.

For optional DNA isolation service from a wide variety of materials, including cell pellets, fresh frozen tissue, blood, plasma refer to our DNA isolation guide.

Deliverables

Raw data as FASTQ files (.fastq.gz)
Additional deliverables for optional bioinformatics, e.g. variant analysis package:
- Alignment file (.bam)
- SNP and InDel tables including annotated variants and effects (.vcf, .tsv, .bed)
- Comprehensive data analysis report (.pdf, .html)
- Tables of copy gain and copy loss events (.tsv)
- Calculation of the TMB tumour mutational burden (.tsv)
- Microsatellite instability status (.tsv)
- View our demo variant analysis report below