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INVIEW OncoRNAfusion - 107

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  • Oncology solutions >
  • INVIEW OncoRNAfusion
 

 

INVIEW OncoRNAfusion - detection of 107 cancer relevant RNA fusion genes.



RNA-Seq ventures into the realm of precision medicine and clinical care and is already an important diagnostic and prognostic parameters in various types of cancer. INVIEW OncoRNAfusion - 107 was designed to detect fusion events of selected genes, which are previously known prone  to be effected by fusion events and are related to cancer. RNA from tissue or FFPE will be reverse transcribed into cDNA following a enrichment process.

 

 

>> Get Quote / Order

>> order Barcodes

>> Industrial-grade

 

Applications

 

  • Detection of well-known tumour-associated fusion events of 107 selected genes
  • Detection of novel fusion events, one fusion partner must be one of the 107 selected genes

 

 

Clinical applications

 

  • Molecular stratification
  • Guidance for treatment decisions
  • Assignment of patients to clinical trials
  • Post-treatment follow-up, especially for monitoring of multiple mutations

Highlights of INVIEW OncoRNAfusion

 

  • Enrichment of 107 genes prone to be effected by fusion events
  • Highly cost-effective
  • Optional flexible Sequencing depth for detection of low expressed genes
  • Individual batch size starting from one sample
 

 

>> FAQ's

>> Sample Submission

>> oncology solutions

 

 

 

 

Product Specifications & Ordering

 

 

Oncology product selection guide

Depending on the project goal, you can select the most suitable INVIEW product from us:

 

INVIEW Exome

Exome for detection of mutations within exomic regions

If you are working with liquid biopsy samples / cell-free DNA, then we recommend the INVIEW Liquid Biopsy Oncoexome.

 

Clinical Research Exome CRE V4
CRE V4 for detection of mutations within exonic regions as well as selected non-coding regions and many more

 

INVIEW Oncoprofiling
Oncoprofiling for cost effective detection of mutationwn within 728 selected genes related to cancer.

If you are working with liquid biopsy samples / cell-free DNA, then we recommend the INVIEW Liquid Biopsy Oncoprofiling.

 

INVIEW OncoRNAfusion
OncoRNAfusion for cost effective detection of fusion events involveing 107 genes prone to be effected by fusion events

Accepted starting material

Accepted starting material 

 

  • Total RNA from various sources
  • rRNA depleted total RNA
  • Amount: recommended: 300 ng for fresh frozen material (400 ng for FFPE samples)
  • Dissolved in RNase-, DNase- and protease-free Tris-HCL buffer (pH 7.5 to pH 8.0)

 

Accepted starting material for RNA isolation

  • Tissue
  • Cells
  • Body fluids
  • FFPE tissue

 

Please note that only S1-classified material is accepted for RNA isolation ordered online. More information about the current classification of biological material can be found here (TRBA 466). Please contact us for further information on isolating RNA from material classified as S2.

 

 

Library preparation & enrichment

  • First and second-strand synthesis
  • Adaptor ligation and indexing
  • Hybridization for enrichment of target sequences
  • Amplification of enriched DNA fragments
  • QC and pooling of final libraries

Sequencing

  • Technology: Illumina
  • Run mode: 2 x 150 bp
  • Data output: 5 M read pairs per sample  (+/- 3%)
  • More data packages per sample also possible

Bioinformatic analysis

Bioinformatic analysis

  • Sequence quality control
  • Mapping & alignment
  • Detection of gene fusion events
  • Variant calling

 

Deliverables

  • FastQ Files (sequences and quality scores)
  • Alignment file (bam)
  • Table of potential RNA fusions
  • List of potential sequence variants
  • Summary report

 
 

 

 

 

 

 

 

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>> READ MORE

 

 

 

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WES and WGS


>> READ MORE

 

 

 

INVIEW
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Sequence all relevant
protein-coding regions


>> READ MORE

 

 

 

EVOCard


The prepaid card for more
flexibility in your research


>> READ MORE

 

 

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