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Benefit from more than 25 years of experience in oligonucleotide synthesis!
Optimise your research and save time with high quality gene synthesis and molecular biology services.
Hiqh quality Sanger sequencing with highest flexibility for every sample type.
NGS from experts - ISO-certified, fully automated and easy to order online.
Cell line authentication, Mycoplasmacheck, Fragment length analysis & tailored projects.
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Our genomics solutions support you along your drug development chain of small and large molecules and in precision medicine.
Benefit from our range of tailored, high throughput genotyping solutions to help you achieve your goals faster, for less.
Welcome to your full-service laboratory. Benefit from our end-to end solutions for sample collection kit logistics and genomic solutions.
DNA-based solutions to improve and support your analysis, monitoring and traceability across your value chain.
Our scalable and high-quality oligonucleotides synthesis offer makes us an ideal partner for your industry applications.
For your research project in academic, governmental and industrial environment we have the right genomic service.
Eurofins GenomicsGermany GmbHAnzinger Str. 7a85560 EbersbergGermany
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Dr. Melvin Siliakus
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Oligonucleotide Synthesis
Gene Synthesis & Molecular Biology
Next Generation Sequencing
Genotyping & Genexpression
Primers & Probes for qPCR Applications
Oligos for Next Generation Sequencing
Primers for Sanger Sequencing
Oligos for Cloning Applications
Custom Oligos
Oligo Tools
Synthetic genes
GeneStrands
Molecular Biology Services
Sanger Sequencing Services
Prepaid Products for Sanger Sequencing
Additional Services
Genome Sequencing
Transcriptome Sequencing
Metagenome / Microbiome
Exome Sequencing & Oncology Solutions
CRISPR & Prepaid NGS Coupons
VIRUS
Plasmid Sequencing
Amplicon sequencing & Ready-to-Load
Oxford Nanopore projects (WGS, Amplicons, 16S)
ONT Lite
Mycoplasmacheck
CLA & FLA
Others
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ONT GridION & Illumina NovaSeq
Hybrid assembly for bacteria combines 30x long-read data with 300x short-read accuracy to enhance consensus quality in homopolymer regions and methylation motifs. This method achieves the most precise and reliable bacterial genome assemblies, often resulting in single-contig replicons for the majority of individual genomes.
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Applications
De novo assembly of bacteria (< 7 Mb) and intrinsic plasmids (no reference sequence required)
Highlights
>> Sample Submission
Product Specifications & Ordering
We are working on an optimised process. Until this process is established we do need 2 samples shipped to us. Both have the same sample requirements:
1st sample
Library preparation (amplification-free, including minimal fragmentation); using v14 library prep chemistry
Sequencing using Oxford Nanopore GridION (flow cell R10.4.1), on average 30x genome coverage
2nd sample
Illumina-compatible PCR-based library preparation using Unique Dual Indexing
Sequencing on Illumina NovaSeq 6000 or NovaSeq Xplus (2 x 150 bp), on average 300x genome coverage
Combined bioinformatics analysis
Assembly Quality Assessment: Thorough evaluation of the draft assembly to ensure accuracy and completeness.
Polishing: Enhancement of the assembly quality through polishing with Illumina data, resulting in a more refined genomic sequence.
Annotation: Detailed annotation of the polished assembly, identifying genes and other functional elements.
For the sample preparation please view "starting material"
For the ordering process please be aware that for now you need to place 2 separate products in your cart for ONT sequencing and Illumina sequencing.
1. ONT Sequencing
For your ONT Sequencing you need the following barcodes:
ONT free barcodes >>
When using these barcodes please submit your ONT samples using this order page:
Bacterial Genome Sequencing on ONT >>
2. Illumina Sequencing
For your Illumina sample you need the following barcodes:
NGS free barcodes >>
When submitting the sample please use the following order page to order the Illumina sequencing and polishing (please select the Bacteria Hybrid assembly product)
INVIEW Resequencing >>
We strongly recommend to use the same sample name for the Illumina and ONT part (discrimation happens via the barcode)
Please note that we are strongly working on a much more convenient solution for you!
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Bacterial genome sequencing
How to sequence your bacterial genome
>> READ MORE
INVIEW Resequencing
Bacterial genome resequencing using Illumina
INVIEW Metagenome
Analyse the metagenomic composition of your sample
Transcriptome Bacteria
Analyse the expression profile of your bacteria
Quality is important for us at Eurofins
Our products and services are produced and performed under strict quality management and quality assurance systems.