NGSelect Ready-2-Load

NGSelect Ready-to-Load

Convenient Illumina sequencing of ready-to-load libraries (RTL).

NGSelect RTL is designed to deliver cost-efficiency and ease of use for customers with ready-to-load library pools (RTLs).

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Applications

Ready-to-sequence libraries provide distinct advantages for researchers who are performing Illumina next-generation sequencing:

Fast results – our large sequencing capacity and automated workflows ensure that you receive your results promptly
High quality – benefit from our vast experience and optimized use of Illumina flow cells
Diverse sequencing strategies including PE150, PE100, PE50 on NovaSeq X series flow cells as well as other customized strategies for projects requiring custom solutions.

Highlights

Breites Spektrum an Sequenzieroptionen: Wählen Sie aus verschiedenen Lesemodi und Read-Anzahlen.
Die Dienstleistungen werden in nach ISO 17025 zertifizierten Laboren in Europa durchgeführt.
Präzise Bestimmung der Pool-Konzentration und entsprechende Verdünnung für die Sequenzierung.
Sortierung anhand Illumina-kompatibler Index-Reads ohne zusätzliche Kosten.
BCL-Dateilieferung auf Anfrage möglich.
Datenübertragung über sicheren FTP-Zugang oder Upload an einen vom Kunden angegebenen Speicherort auf Anfrage.

>> FAQ's RTL

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Product Specifications & Ordering

Starting material

Sample Type: Purified library pool, compatible with Illumina sequencing and index primer *
Volume: minimal 40 µL**
Concentration: > 2.5 nM
Total library size should be fitting to the required read mode (insert size + sequencing adapters):
- 250 bp to 450 bp for Novaseq + PE150 read mode
- 250 bp to 600 bp for MiSeq + PE300 read mode
- Minimum average size: 150 bp
- Maximum average size: 800 bp (MiSeq), 650bp (NovaSeq)
- For custom options please contact us
Buffer: RNase-, DNase- and protease-free Tris-HCl buffer (ph 7.5 - 8.0), EB, or low TE (<0.1 mM EDTA)
Format: barcode labelled 1.5 ml Eppendorf safe-lock tubes
Shipment Method: recommended dry ice

*Ready-to-load library pool must be purified using columns or beads to remove low molecular weight impurities, such as primers, nucleotides and reaction buffer.

**One tube with minimal 40µL per lane is mandatory. E.g. for a complete 10/25B flow cell, a total of 8 tubes are required. Samples to be sequenced on one lane, need to be pre-pooled.

Specifications

QC of library quality and quantity is performed  
Technology: Illumina Novaseq X+, Novaseq 6000 or MiSeq

Platform	Flow Cell Type	Read mode	Lanes per flow cell	Read pairs per lane	Read pairs per flow cell	Service type*
NovaSeq X+ **	25 B	PE150	8	3.25B	26B	standard
	25 B	PE50/PE100	8	3.25B	26B	custom
	10B	PE150	8	1.25B	10B	standard
	10B	PE50/PE100	8	1.25B	10B	custom
	1.5B	PE50/PE100/PE150	2	800M	1.6B	custom
MiSeq		PE250/PE300	1	n.a.	9.6-12.8M	standard
MiSeq		SE50/PE50/PE150	1	n.a.	9.6-12.7M	custom

* Only "standard" service type is compatible with rapid turn-around times. If a "custom" service type is needed, a full flow cell has to be ordered, please be aware that it may require a longer delivery time.

Asymmetric run modes can also be tailored to your specific needs. For projects requiring a different platform or configuration, feel free to reach out to us.

**For NovaSeq 6000 SE50/PE50/PE150/PE250 FC sequencing available on request.

To calculate the total output in bases, multiply the number of read pairs by the read length, and multiply by 2 for paired-end (PE) sequencing. For example, sequencing with a 150 bp paired-end read mode on a 10 billion read flow cell yields approximately 3 terabases (Tb).