NGSelect RNA

RNA-seq approach that fits individual needs.

NGSelect RNA offers a cost-efficient, rapid and flexible approach to Illumina RNA-seq. The product enables researchers to perform RNA sequencing without any additional services. Alternatively, users can outsource other parts of the sequencing workflow, including RNA isolation, rRNA depletion. Customers can choose from a complete selection of cDNA libraries, including non-strand specific libraries, strand-specific cDNA libraries and polyA libraries. All steps of the sequencing workflow have been carefully optimised by our R&D team and samples are professionally processed by trained specialists. The transcriptome analysis can be simplified if outsourced to our BioIT experts, who deliver ready-for-publication results with fast turnaround times.

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Applications

The applications of NGSelect RNA cover most uses of transcriptome analysis. Some of the most standard uses of the product include:

Discovery of novel or rare transcripts
Comparison of gene expression levels between different samples
Alternative splicing analysis
Detection of gene variants, including SNPs and InDels

Highlights

Access to cDNA sequencing capacity on the leading Illumina platform
Choice of different RNA-seq library types, sequencing read modes
Applicable to any organism from prokaryotes to eukaryotes
Individual combinations of different parts of the sequencing workflow: cDNA sequencing, cDNA library preparation, pre-sequencing, including RNA isolation and rRNA depletion and BioIT transcriptome analysis

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Product Specifications & Ordering

Starting material

Starting material

Library Type

Starting Material

Strand-specific cDNA library

Eukaryotes (polyA enrichment): 150 ng total RNA (min 25 µl / optimal concentration 6 - 50 ng/µl (absolute concentration 200 ng/µl))

Eukaryotes & prokaryotes (rRNA depleted / globin depleted):

min 375 ng (min 10 µl, concentration > 37.5 - 200 ng/µl)

*For less starting material, please contact us.

Please note that only S1-classified material is accepted for RNA isolation ordered online. Please contact us for further information on RNA isolation from material classified as S2.

Pre-sequencing options

Dependent on your project layout it is recommended to select a pre-sequencing option to get the most out of your sequencing data.

Here you can see an overview of the different services:

Pre-Sequencing Service	This service includes	You get data for	Comment
RNA-seq without PolyA or Depletion	cDNA library generation of all present RNAs	Total RNA	You receive data for the total RNA including high abundant RNAs like rRNA (~90 % of total RNA) and specific mRNAs
Poly-A-selection*	Bead-based poly-A-selection of your samples (mRNA enrichment)	Only mRNA	This service targets polyA-tails (mRNA) in a total RNA sample. Therefore, only polyA containing fragments will be targeted and sequenced. mRNAs usually make up only 1 to 2 % of the total RNA. If your samples have poor quality (e.g. FFPE material), the mRNA may be degraded. This would result in mRNA fragments without polyA tails which then could not be targeted. If you expect poor quality you might get better data for your research question with selecting rRNA depletion
rRNA depletion	Bead-based rRNA depletion of your total RNA samples (HMR/Bacteria/Plant)	Total RNA minus rRNA	This service targets ribosomal RNA and will remove it from the total RNA. Usually, rRNA make up ~90 % of total RNA. All remaining RNAs, including mRNA, tRNA lncRNAs, etc. will be prepped and sequenced
globin depletion§	Bead-based depletion of globin mRNA of your samples	Total RNA minus globin mRNA	This service targets globin mRNA and will remove it from the total RNA. The remaining RNAs will be prepped and sequenced. This service can be combined with: rRNA depletion or PolyA enrichmen