INVIEW Resequencing

Genome sequencing

Whole Genome sequencing – Non-Human.

IWhole Genome Sequencing (WGS) with Illumina short-read technology offers high-precision sequencing with exceptional accuracy, enabling the detection of single nucleotide variants (SNVs) and small InDels.

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Applications

Resequencing of whole genomes
Identification of genetic variations, single base
mutations, insertions and deletions
Disease pathway analysis
Breeding studies
Production strain optimisation
Metabolic engineering

Highlights

Guaranteed coverage for your samples
- 100 x for bacteria
- 70 x for yeast
- 30 x for all larger genomes (up to 180 Mb)
Flexible solutions for non-human and non-prokaryotic samples, including animals, plants, bacteria, yeast, other eukaryotes, or viruses
Available from one sample
Short turnaround times starting from 12 working days
Unique Dual Indexing (UDI) ensures high-quality data
Highly sophisticated bioinformatics services & interactive analysis report
Optional DNA isolation service
Data transfer via secure FTP

Services

Our standard Variant Analysis service includes detection and annotation of germline single nucleotide polymorphism (SNPs), insertions and deletions (InDel) by comparing your data against a reference genome.
Additionally, we provide optional services such as structural variant analysis and copy number variation detection.
For larger structural variants, short tandem repeat (STR) expansion genotyping, de novo assemblies and analysis of modified base calls please visit our WGS - Non-Human (Long-Read) service.
For optional DNA isolation service, please refer to our DNA isolation guide.
We do not accept samples with higher biosafety level than S2. GMOs are only accepted with S1 leve

Bacteria Genome Sequencing

For high-quality complete assemblies, larger structural variants, analysis of modified base calls or de novo assemblies please visit our Long Read Bacteria WGS.
For Hybrid assemblies of short-read and long-read data for perfect bacterial genome sequences available visit our Hybrid Assembly Bacteria service.

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Specifications

QC of DNA quantity is performed
Illumina-compatible DNA library preparation using Unique Dual Indexing
Technology: Illumina NovaSeq 6000 or NovaSeq X+s
Run type: paired-end
Read length: 2 x 150 bp
Predefined packages (multiples of 5 Mio read pairs), depending on your needs we recommend the following coverage:
- Germline/frequent variant analysis: 30-50x
- Somatic/rare variants: 100-1000x
- De novo assembly: 100-200x 
Additionally a flexible product where you can choose your required data output is also available (click here >>)

Note: Coverage is calculated using the formula

(Read length) × (Total number of reads) ÷ (Genome size).

For example, sequencing a genome of 5 Mb with 5 million reads at 2 × 150 bp read length results in a coverage of 300×.

Starting material

Sample Type: Genomic DNA (RNA-free)
Minimum Amount: 600 ng
Volume: up to 200 µL
Concentration: >3 ng/µl
Purity (OD260/280): 1.8-2.0
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Format: barcode labelled 1.5 ml Eppendorf safe-lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, full-skirted, leave position G12 & H12 empty 
Shipment Method: room temperature

Bioinformatics analysis

Variant analysis: detection and annotation of single nucleotide polymorphism (SNPs) and insertions and deletions (InDels)
De novo genome assembly (only for haploid organisms)