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        • All about biomarker discovery
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        • Plant Breeder
        • DNA marker discovery
        • Marker-assisted selection
        • GRAS-Di®
        • Microbiome and metagenomics
        • Animal Breeder
        • DNA Marker discovery
        • Pathogen screening
        • Parentage testing
        • Genomic selection
        • Marker-assisted selection
        • Webshop - GenFarmEval
        • Favorite Content
        • Microarrays Accelerate Blue Biotechnology
        • How To Do NGS 50% Faster
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        • GenFarmEval.com

          Visit our Webshop for Farmers

          learn more

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        • Pine Nuts – Why Testing For Edibility Matters
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Human Whole Genome Sequencing

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  • INVIEW Whole Genome Sequencing
 

Short Read Human Whole Genome Sequencing

 

Human Whole Genome Sequencing (WGS) with Illumina short-read technology offers high-precision sequencing with exceptional accuracy, enabling the detection of single nucleotide variants (SNVs) and small InDels.

 

>> Get Quote

>> Order Barcodes

 

Highlights

 

  • Short turnaround times starting from 10 working days
  • Unique Dual Indexing (UDI) ensures high-quality data.
  • You can choose between two options: guaranteed reads or a read range for a lower price
  • Highly sophisticated bioinformatics services & interactive analysis reports
  • Optional DNA isolation service
  • Data transfer via secure FTP

Applications & Services

 

  • Our standard Variant Analysis service includes detection and annotation of germline single nucleotide polymorphism (SNPs), insertions and deletions (InDel).
  • Additionally, we provide optional services such as structural variant analysis, copy number variation detection, tumor vs. normal and tumor-only (somatic) variant analysis to pinpoint genetic mutations in tumor samples, joint-genotyping to analyze multiple samples for shared genetic variations, and population genotyping to understand genetic diversity within specific groups.
  • Customizable BioIT solutions to tailor bioinformatics pipelines and tools to meet the unique needs of your research or clinical requirements
  • For larger structural variants, short tandem repeat (STR) expansion genotyping, and analysis of modified base calls please visit our long-read human WGS service.
  • Optional DNA isolation service: from various tissue types and cell lines. 
 

 

>> FAQ's wGS

>> Sample Submission

 

Specifications

 

  • QC of DNA quantity is performed
  • Illumina-compatible PCR-based library preparation using Unique Dual Indexing
  • Technology: Illumina NovaSeq 6000 or NovaSeq Xplus
    • Run type: paired-end
    • Read length: 2 x 150 bp
  • Predefined or flexible read packages (multiples of 5 Mio read pairs)
    • For on average 30x coverage (90 Gb) raw data per package, we recommend 300 Mio read pairs NovaSeq (2 x 150 bp)
  • Depending on your needs we recommend the following coverage:
    • Germline/frequent variant analysis: 030x
    • Somatic/rare variants: 100x

 

 

Product

Premium/Express WGS
Standard WGS

Data output

90 Gb raw data guaranteed 90 GB raw data on average

Starting material

200 ng genomic DNA

Possible with FFPE samples

NO YES

Turnaround time
(starting from)

10 working days 20 working days

Price

$$$ $$

 

 

 More details

 

 

Starting material

  • Sample Type: Genomic DNA (RNA-free)
  • Purity (OD260/280): 1.8-2.0
  • Volume: up to 200 µL
  • Concentration: >2.5 ng/µl
  • Minimum Amount: 200 ng, FFPE 400 ng
  • Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
  • Format: barcode labelled 1.5 ml Eppendorf safe-lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, full-skirted, leave position G12 & H12 empty 
  • Shipment Method: Room temperature

 

Important

Please note that only S1-classified material is accepted for DNA isolation ordered online. More information about current classification of biological material can be found here. Please contact us for further information on DNA isolation from material classified as S2.

 

Deliverables

  • Raw data as FASTQ files (.fastq.gz)
  • Additional deliverables for optional bioinformatics, e.g. variant analysis package:
    • Alignment file (.bam)
    • SNP and InDel tables including annotated variants and effects (.vcf, .tsv, .bed)
    • Comprehensive data analysis report (.pdf, .html)

 

 

 

 

 

 

 

 

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TECHNICAL SUPPORT

Phone: +49 7531 816068

E-Mail: support-eu@genomics.eurofinseu.com

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Fri: 8 : 00 AM – 6 : 00 PM, ET

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E-Mail: support-eu@genomics.eurofinseu.com

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QUOTES, PRICING & SPECIAL REQUESTS

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Please direct inquires about pricing and special project requests to your sales representative.
General questions: support-eu@genomics.eurofinseu.com

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