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INVIEW Metagenome

Metagenome – Next-generation sequencing solution.

This product is ideal for the comprehensive characterization of low-complexity metagenomes and for gaining a broad, unbiased overview of genera within complex microbial communities. The service offers a powerful solution for studying unculturable microorganisms that were previously difficult or impossible to analyze, enabling deeper insights into microbial diversity and function..

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Applications

Analyze the relative abundance of microbial species in their natural habitat.
Profile biological entities, such as viruses, that cannot be distinguished using 16S rRNA sequencing alone.
Monitor shifts in microbial community composition under varying environmental conditions.
Characterize the functional properties of a specific microbiome.

Highlights of the INVIEW Metagenome

Taxonomic profiling of bacteria, archaea and viruses with reduced bias due to independence from taxonomic gene markers such as 16S rRNA
Unique Dual Indexing (UDI) ensures high-quality data.
Optional DNA isolation service
Data transfer via secure FTP

Services

For optional DNA isolation service, please refer to our DNA isolation guide.
We do not accept samples with higher biosafety level than S2. GMOs are only accepted with S1 level.
Our optional basic Metagenome Analysis Service includes sequence quality assessment, removal of poor quality bases, adapters, primers and others, taxonomic profiling using NCBI database of bacterial, archaeal fungal, protozoan and viral genomes and removal of host sequences (only applicable for human, mouse or rat samples)
Our optional premium Metagenome Analysis Service offers a comprehensive suite of analytical tools designed to provide in-depth insights into microbial communities. The analysis uses advanced bioinformatics tools and a comprehensive database of complete genomes to provide a detailed understanding of taxonomic composition, functional potential, and resistance profiles.

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Product Specifications & Ordering

Sequencing specifications

Illumina-compatible DNA library preparation using Unique Dual Indexing
Technology: Illumina NovaSeq 6000 or NovaSeq Xplus
Run type: paired-end
Read length: 2 x 150 bp
Flexible read packages (multiples of 5 Mio read pairs)
Depending on your needs we recommend the following coverage:

Host Genome contamination (%)	Example sample environments	Recommended sequencing depth
<25%	stool, tongue	10 mio read pairs
25-60%	skin	30 mio read pairs
>60%	saliva, nostrils	50 mio read pairs

Starting material

Sample Type: Genomic DNA (RNA-free)
Minimum Amount: 600 ng
Volume: 20 to 200 µL
Concentration: >3 ng/µl
Purity (OD260/280): 1.8-2.0
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Format: barcode labelled 1.5 ml safe-lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, full-skirted, leave position G12 & H12 empty
Shipment Method: room temperature