Next Generation Sequencing - Powered by Innovation & Expertise

INVIEW Transcriptome Bacteria - Gene expression profiling of prokayotes

Obtain high quality mapping results and gene expression profiles for your prokaryotic samples.

Our strand-specific RNA-seq services provide in-depth analysis of bacterial gene expression, uncovering key insights into microbial behavior and regulation. With high accuracy and resolution, we help you accelerate your research and discoveries in bacterial genomics.

In addition to RNA sequencing, we offer RNA isolation services and bacterial rRNA depletion

>> Get Quote / Order

>> Order Barcodes

>> Redeem NGS Coupons

Applications

Ideally suited for all prokaryotes
Analysis of transcript levels from different samples
Mapping studies of bacteria

Highlights

Strand-Specific RNA-Seq
Short turnaround times starting from 15 working days
Unique Dual Indexing (UDI) ensures high-quality data.
Guaranteed data output, starting from 5M read pairs packages
Highly sophisticated bioinformatics services & analysis reports
Data transfer via secure FTP

>> FAQ's Transcriptome

>> NGS Prepaid Coupons

>> Sample Submission

Product Specifications & Ordering

Our standard RNA-Seq analysis pipeline provides a comprehensive approach to understanding gene expression, beginning with quality assessment of sequencing data. We align reads to a reference genome, quantify transcripts, and normalize gene counts, followed by principal component analysis (PCA) to visualize sample relationships and identify expression patterns. Finally, we identify differentially expressed genes through significance testing, generating detailed reports and visualizations—including heatmaps, volcano plots, and various QC metrics—to empower researchers with meaningful biological insights.

Optional RNA-Seq analysis packages:

Variant detection and its effect on protein alteration: This analysis is available for single sample onwards and provides a detailed view of the variants that occur in the samples and their effect on protein function.
Gene Set Enrichment Analysis (GSEA): This advanced bioinformatics analysis service utilizes Gene Set Enrichment Analysis (GSEA) to identify biological pathways and gene sets that are significantly enriched in differentially expressed genes. This analysis provides a deeper understanding of the biological processes and pathways that are affected by the changes in gene expression.

For optional RNA isolation service, please refer to our “Sample preparation and shipping guide for Extraction”. 

We do not accept samples with higher biosafety level than S2. GMOs are only accepted with S1 level.

Specifications

Entry QC of RNA quality and RNA quantity
Illumina-compatible stranded RNA-Seq library preparation using Unique Dual Indexing
Technology: Illumina
ina NovaSeq 6000 or NovaSeq Xplus
Run type: paired-endRead length: 2 x 150 bp
Guaranteed data output for predefined 10 Mio read pairs or flexible read packages (multiples of 5 Mio read pairs). We recommend 10 million read pairs for bacterial genomes.

Starting material

Sample Type: total RNA (DNA-free)
Purity (OD260/280): 1.8-2.2
Recommended RIN: ≥5.0
Resuspension Buffer: Nuclease-free water or Tris-HCL buffer (pH 7.5 to pH 8.0)
Format: barcode labelled 1.5 ml safe-lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, full-skirted, leave position G12 & H12 empty 
Shipment Method: Dry ice, contact us about our recommendation for RNA Stabilization Tubes to ship RNA samples at ambient temperature.

RNA selection method	target RNA	Minimum amount	Minimum volume	Concentration
none	Total RNA	20 ng	10 µl	2 - 20 ng/µl
rRNA depletion	mRNA	375 ng	10 µl	37.5 - 200 ng/µl

Here you can see an overview of the different services:

Pre-Sequencing Service	This service includes	You get data for	Comment
RNA-seq without PolyA or Depletion	cDNA library generation of all present RNAs	Total RNA	You receive data for the total RNA including high abundant RNAs like rRNA (~90 % of total RNA) and specific mRNAs
rRNA depletion	Bead-based rRNA depletion of your total RNA samples (HMR/Bacteria/Plant)	Total RNA minus rRNA	This service targets ribosomal RNA and will remove it from the total RNA. Usually, rRNA make up ~90 % of total RNA. All remaining RNAs, including mRNA, tRNA lncRNAs, etc. will be prepped and sequenced