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RNA-Seq – Ultra Low

Our Ultra Low RNA-seq services enable precise mRNA analysis from eukaryotic RNA samples with extremely low concentrations down to 0.12 ng/µL. Get reliable, high-resolution results even with limited material, accelerating your discoveries in genomics research. Recommended for sequencing cells after fluorescence-activated cell sorting (FACS) or immunoprecipitation..

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Applications

Explore the distinct biology of different cells within an organ or a tumor
Understand subpopulation responses to environmental stimuli
Enhance study of cell function and heterogeneity in time-dependent processes such as differentiation, proliferation, and tumorigenesis

Highlights

Unique Dual Indexing (UDI) ensures high-quality data.
Guaranteed data output, starting from 5M read pair packages
Trimming of sequencing data included
Highly sophisticated bioinformatics services & analysis reports
Data transfer via secure FTP

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Product Specifications & Ordering

Specifications

Entry QC of RNA quality and RNA quantity
Oligo-dT primed cDNA generation
Illumina-compatible RNA-Seq library preparation using Unique Dual Indexing (non-stranded)
Technology: Illumina NovaSeq 6000 or NovaSeq Xplus
Run type: paired-end
Read length: 2 x 150 bp
Guaranteed data output for predefined or flexible read packages (multiples of 5 Mio read pairs), depending on your needs we recommend the following coverage:

Starting material

Sample Type: eukaryotic total RNA (DNA-free)
Volume: minimum 10 µL 
Concentration: >0.12 ng/µl 
Recommended RIN: >6.5
Purity (OD260/280): 1.8-2.2
Resuspension Buffer: Nuclease-free water or Tris-HCL buffer (pH 7.5 to pH 8.0)
Format: barcode labelled 1.5 ml safe-lock tubes; or for > 48 samples in Eppendorf twin.tec PCR Plate 96, full-skirted, leave position G12 & H12 empty 
Shipment Method: Dry ice
Batch size: Minimum 24 samples / batch