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RNA Sequencing

RNA sequencing

Eurofins provides comprehensive RNA-Seq services that offer deep molecular insights into the transcriptomes of a variety of organisms. Our solutions enable precise profiling of all RNA species, including non-coding RNAs, and facilitate the analysis of both sense and antisense transcripts through the use of strand-specific libraries. Whether you're investigating gene expression levels, transcript structures, or other aspects of the transcriptome, our RNA-Seq services deliver high-quality, quantitative results.

In addition to RNA sequencing, we offer RNA isolation services and a variety of library preparation protocols tailored to your specific project needs. These include stranded poly(A) selection methods and rRNA depletion for FFPE, poor-quality, and bacterial samples. For blood RNA, we provide globin depletion, and for projects with extremely limited RNA input, we offer special protocols that can work with as little as 120 pg/µL of RNA. With these flexible and optimized approaches, we ensure reliable, high-quality data for your research.

INVIEW Transcriptome Bacteria

Illumina NovaSeq

Obtain high quality strand specific mapping results and gene expression profiles for your prokaryotic samples.

INVIEW Transcriptome Discover

Illumina NovaSeq

Find strand-specific RNA-Seq solutions for eukaryotic samples, including animals, plants, yeast or viruses

INVIEW Transcriptome Ultra Low

Illumina NovaSeq

Ultra-low input RNA sequencing provides gene expression analysis of samples containing just a few cells.

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Features

Services are performed in ISO17025 certified labs in Europe
Fast turn-around times
Flexible data quantity options
Optional RNA extraction services available
Data transfer via secure FTP

Wide Range of applications & services

Explore our comprehensive bioinformatics services designed to meet diverse research needs.

Our offerings include data analysis, interpretation, and visualization tailored to your specific projects.

Benefit from interactive analysis reports that provide clear insights, enabling you to make informed decisions quickly.

Our expert team is here to support you every step of the way. Elevate your research with our cutting-edge bioinformatics solutions today!

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Product Details

Product	Bacteria	Discover	Ultra Low
Library Type	Strand-specific cDNA libraries	Strand-specific cDNA libraries	Strand-specific cDNA libraries
Starting material	Prokaryoten	Application for total RNA from various sources	Eukaryoten
Sequencing technology	Illumina	Illumina	Illumina
Read Mode	150 bp paired-end reads	150 bp paired-end reads	150 bp paired-end reads
Number of guaranteed reads	Multiples of 10M read pairs	Multiples of 30M read pairs	Multiples of 5M read pairs
Delivery time	15 working days for up to 192 samples	15 working days for up to 192 samples	From 27 working days
Min sample number	From 1 sample	From 1 sample	From 24 samples

Which product to choose

Product	Bacteria	Discover	Ultra Low
Novel transcripts	-	+	-
Strand specificity information	+	+	+
Rare transcripts	Illumina	Illumina	Illumina
Read Mode	Flexible increase of data output	Flexible increase of data output	Flexible increase of data output
Splice variants	n.a.	+	-
Transcript identification	+	+++	+
Differential expression	+	+++	+
Mapping accuracy	Sufficient	Optimal	Sufficient
High quality reference	Mandatory	Additional	Mandatory

Pre-sequencing options

Dependent on your project layout it is recommended to select a pre-sequencing option to get the most out of your sequencing data.

Here you can see an overview of the different services:

Pre-Sequencing Service	This service includes	You get data for	Comment
RNA-seq without PolyA or Depletion	cDNA library generation of all present RNAs	Total RNA	You receive data for the total RNA including high abundant RNAs like rRNA (~90 % of total RNA) and specific mRNAs
Poly-A-selection*	Bead-based poly-A-selection of your samples (mRNA enrichment)	Only mRNA	This service targets polyA-tails (mRNA) in a total RNA sample. Therefore, only polyA containing fragments will be targeted and sequenced. mRNAs usually make up only 1 to 2 % of the total RNA. If your samples have poor quality (e.g. FFPE material), the mRNA may be degraded. This would result in mRNA fragments without polyA tails which then could not be targeted. If you expect poor quality you might get better data for your research question with selecting rRNA depletion
rRNA depletion	Bead-based rRNA depletion of your total RNA samples (HMR/Bacteria/Plant)	Total RNA minus rRNA	This service targets ribosomal RNA and will remove it from the total RNA. Usually, rRNA make up ~90 % of total RNA. All remaining RNAs, including mRNA, tRNA lncRNAs, etc. will be prepped and sequenced
globin depletion	Bead-based depletion of globin mRNA of your samples	Total RNA minus globin mRNA	This service targets globin mRNA and will remove it from the total RNA. The remaining RNAs will be prepped and sequenced. This service can be combined with: rRNA depletion or PolyA enrichment, a minimum of 48 samples per project is required
Ultra low	cDNA library generation of all present RNAs with an adapted protocol for samples with low RNA concentration	Total RNA	This service is intended for samples with a RNA concentration of 0.12-2.3 ng/µl. Because this service needs special handling, a minimum of 48 samples is required.